APPLICATIONS OF LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY

High-Performance Liquid Chromatography (HPLC)

Abstract

It is a sophisticated analytical technology with very high sensitivity and specificity, the Liquid 
Chromatography-Mass Spectrometry. Combining Liquid Chromatography (LC) with 
spectroscopy is known as LC-MS (MS). It is possible to separate components using the Liquid 
Chromatography (LC) technique, which is followed by the transfer of the samples to Mass 
Spectrometry (MS) where the detection, identification and determination of masses may be done 
in the presence of other elements. Quantitative and qualitative analysis of pharmaceutical drug 
ingredients, intermediates, and related chemicals is accomplished using LC-MS. in-vitro 
dissolution, bio-equivalence, bioavailability, and metabolite investigations are the most common 
uses of LC-MS. It is also utilised in fundamental research, agriculture, forensics and the food and 
beverage sectors. LC-MS Instrumentation and uses of the LC-MS method are briefly reviewed in 
this article.